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. 2009 Dec 1;23(23):2778–2791. doi: 10.1101/gad.541309

Figure 7.

Figure 7.

KT localization depends on Ndc80 but not on Mad2 or Bub1. (A) Localization of Stu1 to detached KTs depends on Ndc80. GAL-NDC80 cells were depleted of Ndc80 during a G1 arrest, and Stu1 localization was quantified 105 min after the release. n > 100. Bar, 2 μm. (B) Localization of Stu1 to detached KTs does not depend on Mad2 or Bub1. Ninety minutes after the release from G1 into nocodazole, spindle poles (Spc72-3mCherry), KTs (Ame1-GFP), and Stu1-CFP were visualized, and Stu1 localization was quantified. n > 100.