Figure 3.

Tcr protein is retained in the spermatozoa expressing the transgene. (A,B) Ultrathin sections of spermatozoa derived from the cauda epididymis of Tg9/0 or wild-type control (wt) males assayed for Myc-Tcr protein using immunogold-labeled anti-Myc antibodies reveals the localization of Tcr protein at the fibrous sheath (FS) and the ODFs of the principal piece of the flagellum (A) and in sperm nuclei (B). A fraction of Tg9/0-derived sperm nuclei show no or few immunogold particles, like wild-type sperm. White boxes indicate areas shown at higher magnification on the right; for better visualization, immunogold particles are shown as white spots on duplicate panels. (Ax) Axoneme. Bars: A, 500 nm; B, 280 nm. (C) Histograms showing the number of sections of sperm nuclei corresponding to the number of immunogold particles counted, based on the analysis of 202 and 204 sperm nuclei derived from wild-type (wt) or Tg9/0 animals, respectively. In sperm nuclei of hemizygous Tg9/0 males, one part of the distribution is concentrated at low immunogold particle counts, whereas the remainder is widely spread at higher counts (heavy tail distribution).