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. 2009 Dec 14;4(12):e8246. doi: 10.1371/journal.pone.0008246

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Andreani et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MDM were infected with HIV in presence of T. cruzi blood trypomastigotes (A) or trypomastigotes free-supernatant (TcSn) (B) in three different schemes: HIV 24 h after T. cruzi (T. cruzi-HIV), HIV 24 h before T. cruzi (HIV-T. cruzi) or HIV at the same time as T. cruzi (HIV+T. cruzi), where T. cruzi indicates either trypomastigotes or TcSn. P24 antigen production was measured at days 4, 8 and 12 p.i. in culture supernatants. Results are expressed as mean ± SD of triplicates of infection and a representative experiment of at least 3 independent experiments performed with cells from different donors is shown.