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. 2009 Dec 11;4(12):e8262. doi: 10.1371/journal.pone.0008262

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Sasaki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Self-renewal assay. The ratio of alkaline phosphatase positive colonies is shown after normalization against the ratio obtained with control cells (value = 1). Approximately 70% of the colonies derived from the control cells remained in an undifferentiated state in feeder-free culture. The values shown are the means±SD from three independent experiments and significant values are indicated; *P<0.01, in comparison to the control. (B) Real time PCR analysis of undifferentiated state markers in the cells 4 days after transfection. The results are shown after normalization against the values obtained for control cells (value = 1). The values shown are the means±SD from two independent experiments. (C) Proliferation assay. The ratio of proliferation 48 h after culture is shown after normalization against the values obtained with control cells (value = 1). The values shown are the means±SD from three independent experiments and significant values are indicated; *P<0.01, in comparison to the control.