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(A)–(D) Real time PCR analysis of germ layer markers 4, 8, and 12 days after EB formation (A, neurectoderm marker; B, mesoderm marker; C, primitive ectoderm marker; D, extraembryonic endoderm (ExE) marker). The results are shown after normalization against the values obtained with control EBs on day 4 (value = 1). The values shown are the means±SD of two independent experiments.
