Table 1. The apparent association (ka), dissociation (kd) rate constants and equilibrium dissociation constants (KD) for the interaction of BMP4 and Wnt3a with immobilized heparin or CS-E.
Ligand | GAG | ka (M−1Sec−1) | kd (Sec−1) | KD (nM) |
BMP4 | Heparin | 2.76×105 | 1.92×10−2 | 69.4 |
BMP4 | CS-E | 1.44×105 | 4.33×10−3 | 30.0 |
Wnt3a | Heparin | 2.22×105 a | 5.77×10−3 a | 26.0 a |
Wnt3a | CS-E | 8.26×105 | 2.26×10−2 | 27.3 |
The ka, kd and KD values were determined by SPR analysis.
The ka, kd and KD values of Wnt3a against heparin have been published in our previous paper [17].