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. 2009 Dec 14;4(12):e8302. doi: 10.1371/journal.pone.0008302

Figure 5. PMA induces binding of a Stat1-nucleolin complex to the GAS element.

Figure 5

THP-1 cells were treated with IFNγ for 2 h or with PMA for 24, 48 or 72 h. Whole cell extracts were prepared and used for EMSA. Subunit composition of DNA binding complexes were analyzed by antibody competition with antibodies directed against Stat1 and nucleolin, as indicated. Complex specificity was verified with oligonucleotide competition, using 5-fold excess of GAS or H2K, respectively. White arrow indicates Stat1 homodimers; black arrow indicates a Stat1-nucleolin complex.