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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Pediatr Res. 2009 Apr;65(4):414–419. doi: 10.1203/PDR.0b013e318199093c

Figure 4.

Figure 4

Protein levels of calcium handling proteins in the developing human ventricle. A. Example of western blots for NCX (Na/Ca exchanger). Lanes are labeled with age of patient (d=days, m=months) with newborns furthest left, young infants in the center and older infants furthest right. B. Density of NCX plotted as a function of age (newborns, solid triangles; young infants, open circles; older infants, solid circles), showing an inverse linear relationship (R=−0.66, p=0.003) between NCX protein levels and age. C. Average NCX protein levels for the three age groups: newborn (open, n=6), young infants (hashed and shaded, n=6) and older infants (shaded, n=6). *, p<0.05. D. Western blots of SERCA (SR Ca-ATPase pump) labeled with ages as in (A). E. Density of SERCA plotted as a function of age ((R=−0.11, p=n.s.). F. Average SERCA levels for the three age groups (n=6 for each age). Parts A–F used membrane proteins. G. Western blots using total proteins of SERCA, phospholamban (PLB) and GAPDH for loading control. H. Summary data for part G, normalized to GAPDH (n=6 newborn and n=5 infant).