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. 2009 Dec 3;106(51):21930–21935. doi: 10.1073/pnas.0811794106

Fig. 5.

Fig. 5.

Western blots for proteins relevant to neuronal cell growth. (A) The level of total protein PP2Ac (phosphatase 2A, catalytic subunit) increase in all three cultured states of the TO cells (P = 0.0281, 0.0012, and <0.0001 for P, 3D, and 5D cells, respectively). (B) The level of demethylated PP2Ac also increased in TO cells, with P = 0.0012, 0.0082, and 0.0006 for P, 3D, and 5D cells, respectively. (C and D) No difference of total ERK1/2 (C) could be observed between the TO and the OT cells; phospho-ERK1/2 level (D) was significantly reduced in TO cells (for total ERK1/2, P = 0.4226, 0.3992, and 0.8563 and for phospho-ERK1/2, P = 0.01181, P < 0.0001, and P < 0.0001 for P, 3D, and 5D cells, respectively). (E and F) for both total p38 (E) and phospho-p38 (F), an increase was visible at the 5D stage of the cells (for p38, P = 0.1087, 0.6742, and 0.02038 and for P-p38, P = 0.2513, 0.6881, and 0.0013, respectively for P, 3D, and 5D cells). (G) The total amount of cyclin-dependent protein kinase 2 (CDK2) appeared reduced in TO cells in differentiated cells (for P, 3D, and 5D cells: P = 0.2032, 0.0005, and 0.0004, respectively), reflecting the growth retardation associated with the TO cells. (H) A significant decrease was detected in cyclin E at 3D (for proliferating cells, P = 0.394; for 3D cells, P = 0.0283; for 5D cells, P = 0.709). (A–H) Typical result out of three runs performed in three repeats. Densitometric analysis was normalized to an arbitrary value of 1.0 that represented the maximal value recorded within each experiment series. Mean and SE are indicated.