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. 2009 Dec 3;106(51):21924–21929. doi: 10.1073/pnas.0909894106

Fig. 1.

Fig. 1.

Overexpression of cyclin E1 and cyclin D1 influence cell-cycle characteristics of cortical precursors. (A) Cell-cycle duration (Tc) of control and GOF precursors measured by time-lapse videorecording, on E14.5 organotypic slices, 24 h after electroporation. Overexpression of cyclin E1 and cyclin D1 leads to a significant shortening of Tc (22.8 h in control; range: 15–28 h, n = 16) vs. 19.75 h in GOF precursors (GOF cyclin E1: range: 13–23 h, n = 13; GOF cyclin D1: range: 13–22 h, n = 25). Unpaired t test: cyclin E1 GOF: P = 0.0024; cyclin D1 GOF: P = 0.0075. (B) TG1 in GOF and control transfected precursors. A small proportion of EdU+/EGFP+ cells are detected in both GOF conditions, 8 h after electroporation, whereas the first EDU+/EGFP+ precursors (3.3%) are detected only 12 h after electoporation in control precursors. (C) Diagram: Electroporation transfects a cohort of cells in late S, G2/M phases (cells in green). The EGFP+ cohort progresses through G1 phase at different rates between control and GOF conditions. Only GOF precursors have reached S phase 8 h after electroporation.