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. Author manuscript; available in PMC: 2009 Dec 3.
Published in final edited form as: Mol Immunol. 2007 Feb 2;44(10):2598–2604. doi: 10.1016/j.molimm.2006.12.011

Fig. 2. VV binding to IgA1 and IgA2 myeloma proteins using ELISA (A) and western blot analysis (B). The numbers identify the same proteins in both figures.

Fig. 2

Fig. 2

A) VV binding to myeloma proteins (0.625 μg/ml) determined by capture ELISA. Protein were native (black bars) or treated with neuraminidase to remove sialic acid (light bars).

B) One μg IgA1 and IgA2 protein were loaded per well. Selected western blot membranes were treated with neuraminidase (NA), then developed with VV. IgA myeloma proteins used: (1) Mce, (2) Ham, (3) Ste, (4) Gou, (5) Lat, (6) Ber1, (7) Sin, (8) Kni, (9) Fel, (10) Ber2, (11) Hum, (12) Kur, (13) Cur, and (14) Cob. An IgG myeloma protein (Mat) was used as a negative control. Anti-IgA staining was performed as a loading control.