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. Author manuscript; available in PMC: 2010 Dec 7.
Published in final edited form as: Cell Stem Cell. 2009 Dec 4;5(6):646–658. doi: 10.1016/j.stem.2009.09.015

Fig. 2.

Fig. 2

Lmx1a directly regulates Wnt1 expression. a. qPCR analysis on in vitro differentiated ES cells with empty or Lmx1a-expressing retrovirus (ND3; n=4, p<0.05). b–c. Immunocytochemistry on the same cells. Scale bar represents 50μm. Inset shows Hoechst staining. d. ChIP-qPCR analysis on Wnt1 and Wnt5a promoter region (n=3, p<0.05). In vitro differentiated ES cells transduced with retrovirus expressing HA-tagged Lmx1a was fixed for ChIP at ND3. ChIP fragments were immunoprecipitated either with normal rabbit IgG or anti-HA antibody and analyzed by qPCR. Results represent the average of three independent ChIP experiments.