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. Author manuscript; available in PMC: 2010 Dec 7.
Published in final edited form as: Cell Stem Cell. 2009 Dec 4;5(6):646–658. doi: 10.1016/j.stem.2009.09.015

Fig. 6.

Fig. 6

The Wnt1 signaling pathway induces mDA differentiation of ES cells synergistically with the SHH pathway. a. qPCR analysis on in vitro differentiated cells transduced with empty, FoxA2-, Lmx1a-, or Otx2-expressing retrovirus at ND6 (n=4, p<0.05). FLO designates cells transduced with all three viruses that express FoxA2, Lmx1a or Otx2. b–c. Co-transduction of three factors (FLO) leads to a significant increase in Pitx3+ TH+ mDA neurons compared to empty virus-transduced cells. d–g. Cell transduction with three factors does not significantly alter the proportion of neurons (Tuj1+) or astrocytes (GFAP+). h–o. Three factor transduction increases the cells with mature DA phenotype, shown by coexpression of DAT and DDC with TH. p–w. Three factor transduction increases cells with mDA phenotype, shown by coexpression of Lmx1b and Nurr1 with TH. Immunocytochemistry on in vitro differentiated ES cells at stage ND6. Inset shows Hoechst staining. Scale bar represents 50mm.