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LPA1+/- and LPA2+/- mice show reduced neutrophils infiltration to BAL fluids. 18:1LPA (5 μM in 25 μl PBS) were intratracheally injected to wild type, LPA1+/-, and LPA2+/- mice (n = 4-5) for 6 h. BAL fluids were collected and percentage of neutrophils in total cells were examined by Cytospin.
