Table 2.
Quatification of LPA molecular species in BAL fluids
| LPA molecular species | Wt sensitization only (pmol/ml) | Wt sensitization and SEA challenged (pmol/ml) |
|---|---|---|
| 14:0-LPA | 5.6 ± 3.1 | 14.6 ± 1.2 |
| 16:1-LPA | 50.4 ± 37.0 | 160.9 ± 3.7 |
| 16:0-LPA | 88.4 ± 51.7 | 253.6 ± 10.8 |
| 18:2-LPA | 83.9 ± 54.6 | 274.4 ± 7.0 |
| 18:1-LPA | 154.3 ± 114.7 | 517.6 ± 17.9 |
| 18:0-LPA | 54.9 ± 27.4 | 153.7 ± 8.0 |
| 20:5-LPA | 26.7 ± 22.0 | 95.7 ± 1.9 |
| 20:4-LPA | 212.9 ± 168.2 | 739.0 ± 23.1 |
| 20:3-LPA | 51.7 ± 39.8 | 165.2 ± 5.5 |
| 20:2-LPA | 4.7 ± 3.4 | 14.1 ± 0.8 |
| 22:6-LPA | 189.4 ± 114.4 | 548.9 ± 18.1 |
| 22:5-LPA | 291.6 ± 166.2 | 576.9 ± 22.2 |
| 22:4-LPA | 16.8 ± 9.5 | 41.1 ± 1.5 |
| 22:3-LPA | 0.7 ± 0.6 | 1.9 ± 0.5 |
| 22:2-LPA | 0.2 ± 0.1 | 0.2 ± 0.1 |
| Total LPA | 1254.3 ± 357.0 | 3557.9 ± 109.3 |
BAL fluids were collected and lipis were extracted. LPA molecular species were quantified by LC-MS/MS with 17:0LPA as standard.