Table 1.

Effect of different detergents on the release of PhaC1, PhaC2 and other proteins from PHA granules.

Extractant (w/v)a	PhaC1	PhaC2	PhaF	PhaI
	Proteins released from PHA granules (%)b
Tris-HCl pH 8 (100 mM)	n.dc	n.d	n.d	n.d
Phospholipids (0.5%)	n.d	n.d	n.d	n.d
Hecameg (0.5%)	50%	n.d	n.d	10%
Triton X-100 (0.5%)	100%	0-20%	10%	10%
CHAPS (0.5%)	100%	0-20%	n.d	25%
Igepal-CA630 (0.5%)	100%	0-20%	n.d	25%
Rhamnolipids (0.1%)	100%	100%	75%	75%

a) Detergents were dissolved in 100 mM Tris-HCl (pH 8).

b) Percentages of protein release were determined by comparing granule pellet fractions and supernatant fractions on SDS-PAA gels.

c) Not detected (< 2% of total granule bound protein).