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. 2009 Nov 27;5:5. doi: 10.1186/1745-7580-5-5

Figure 4.

Figure 4

Statistical approach to identify translationally-regulated mRNA molecules in LPS-activated moDCs. The 54'675 probe sets present on the Affymetrix U133 Plus 2.0 GeneChip array were first filtered on flags (P in 50% 0 h or P in 50% 4 h or P in 50% 16 h) to obtain a preliminary list of 20'438 probe sets. A 2-way ANOVA analysis with repeats on time (false discovery rate= 0.05) was then performed on the 20'438 selected probe sets, to identify the 375 translationally-regulated mRNA molecules with statistically significant interaction (red circle in Venn diagram). An example of interaction (the two curves are parallel between 0 h and 4 h and are "interacting" between 4 h and 16 h post-LPS) between Total and Polysomal mRNA is indicated on the simplified graph on left, where the different time points of the two RNA groups are connected by their mean gene expression signals. The number of probe sets belonging to each parameter is indicated in brackets. See Additional file 4 for the complete list of translationally-regulated mRNA molecules. Groups defined within each parameter, Time: 0 h, 4 h and 16 h post-LPS; RNA type: Total mRNA and Polysomal mRNA.