Table 2.
Primers for amplification of the fragments containing SNP markers.
| SNP | Forward primer sequence (5' → 3') | Reverse primer sequence (5' → 3') | bpa |
|---|---|---|---|
| Gene MLH1 | |||
| c.1- 93G>A | CAACCCACAGAGTTGAGAAATTT | GCTCAACGGAAGTGCCTT | 152 |
| c.453+79A>G | TGGAAAACTGAAAGCCCC | TTAGAGGATATCTTGGGACCTCC | 201 |
| c.655A>G | CGTGGACAATATTCGCTCC | CTAAAGCAAACTCTTAACACACATAATATC | 156 |
| c.790+955C>A | CCAGCTGTAAATGTTTCAAAATAT | AACATTTTCTGAGCCTTGATTAAA | 200 |
| c.791- 1406C>T | CAGAGTACAGTGACATTAATGAT | TCTCTCTGAACAGATTTAGATGA | 183 |
| c.791- 488A>G | TGATACCATTTCTGTGCCATTTT | GGCCAGCACTATTATAGACGT | 157 |
| c.1038+86T>C | GTCAGGGCGCTTCTCATC | GGAACAACAGCACAATACCTT | 173 |
| c.1039- 78A>G | ATAATTTTAAGATTATAAGGATTTTG | GTGGAGAGACTCAGAATAAGA | 186 |
| c.1668- 19A>G | ACAGCCAGGCAGAACTATTTC | TGAGTATCTGGTAGAACAGTTCTTC | 153 |
| c.1990- 121C>T | CCTGCTCTCATCCCCACT | ACCTGGTCCAAAGAAATTCA | 178 |
| Gene MSH2 | |||
| c.1076+1681G>T | AACGATATTGGAATGATTGGATC | ACTTGCAAATTACCCAGTCCTT | 177 |
| c.1077- 80G>A | AAATGGAATTTTGAGCTGATTT | ATCTTCTACAAAAGCTTCCACTAA | 134 |
| c.1276+1349T>A | GTTGTTATGTCTAATACATAAAGC | TCAAAACGTATGTTTTAATGGTTC | 157 |
| c.1276+1394A>T | GTTGTTATGTCTAATACATAAAGC | TCAAAACGTATGTTTTAATGGTTC | 157 |
| c.1277- 212T>A | CATGTTTCTGCATCTATATTACTT | TAAACAAGCATCATTTGATCCAAA | 201 |
| c.1277- 118G>A | CATGTTTCTGCATCTATATTACTT | TAAACAAGCATCATTTGATCCAAA | 201 |
| c.1386+719T>C | TCAGTCTTAGCCTCCCAAAAT | CGACAACTATTTAGGAGATGCA | 131 |
| c.1387- 914A>G | AGTCTCAGTATGTCACCTAGG | TAGCAGTGCGCACCTGTAGT | 131 |
| c.1511- 91G>T | CAAGTAAGTAGTATTTGAATCTTTTCTG | GCACTGGAATCCAGTTTAATCT | 202 |
| c.1511- 9A>T | CAAGTAAGTAGTATTTGAATCTTTTCTG | GCACTGGAATCCAGTTTAATCT | 202 |
| c.1661+12G>A | TCCAGAAGAATGGTGTTAAATTT | TTAGTAAAACTTATCATAGAACATTCAC | 120 |
| c.1759+107A>G | GGCTTTTGGTAACAGAAGAAAAA | CTGCCATTTTTTGTTTCTATGTG | 176 |
| c.1759+183G>A | GGCTTTTGGTAACAGAAGAAAAA | CTGCCATTTTTTGTTTCTATGTG | 176 |
| c.1760- 1207C>T | TAATTAGGTCTGCTTGGCCATT | CAGAACATTCAAGATAAAAGCATT | 166 |
| c.2006- 6T>C | ACATCTTTGGGCAGGCTG | CCATGAGTACTATCACCCCA | 214 |
| c.2210+175G>A | GTCTTAGTTTAATAGTTGTTTTCC | ATTCCAGGAAGTGTGAACAGT | 198 |
| c.2210+274T>G | GTCTTAGTTTAATAGTTGTTTTCC | ATTCCAGGAAGTGTGAACAGT | 198 |
| c.2635- 214T>C | CCAGTATTCTTTGTAAACCTTGA | GTCTCAAACTCCTCCCACATT | 156 |
a size of amplicon in base pairs (bp); Note: several SNPs are located on the same amplicon