Fig. 1.
Activation of outwardly rectifying Cl- current by acidic pHe. Representative current traces obtained from primary keratinocytes (A) and HaCaT cells (B) by step-like pulses. The membrane voltage was held at -40 mV, and incremental step-like pulses from -100 to 100 mV (20 mV intervals, 400 ms duration, see inset). The currents (B-a) were obtained with Nagluconate solution in the bath (pH 4.2). (B-b) Representative current traces obtained by step-like pulses under different pHe. The current to voltage relations (I-V curves), measured at the end of each pulse, are shown in right panels. Step like pulses were applied in different conditions of extracellular anions (pH 4.2 Br-, and I-). Current traces presented in (C) were obtained from the same cell, respectively. I-V curves of ICl,pH obtained in NaCl, NaBr, and NaI solutions are shown in the right panel of (C) Each symbol represents mean±SEM of current amplitudes normalized to cell capacitance (pA/pF).