Fig. 4.

Ca²⁺-activated Cl^- current (I_Cl,Ca) of HaCaT cells and effects of acidic pH_e. (A) Outward currents with slowly activating kinetics were induced by dialyzing cells against Ca²⁺-clamped CsCl solution (see inset). Lowering pH_e to 7.0 and 6.8 increased the amplitude of outward current. After testing pH_e effects, the inhibition of outward current by 500 µM DIDS was confirmed at pH_e 6.8. (B) Summary of the effects of pH_e on I_Cl,Ca of HaCaT cells. In each cell, the current amplitudes measured at the end of step-pulse were normalized to the control amplitude (pH_e 7.4), and mean±SEM values were plotted (n=8). ^*indicate p-value<0.05.