Fig. 4.

Effect of fasting on pituitary growth hormone (GH)-releasing hormone receptor (GHRH-R) (A), GH secretagogue receptor (GHS-R) (B), and GH (C) mRNA levels in adrenalectomized or shamoperated animals. Animals were fasted for 24 h, 48 h, 72 h or supplied food ad libitum (fed). Both groups were allowed free access to water or 0.9% NaCl solution (adrenalectomized group). GHRH-R, GHS-R, and GH mRNA levels were measured by real-time RT-PCR and adjusted with β-actin. The graph is expressed as a percentage of fed controls. Data are mean±S.E.M., n=5 animals/group. ^*, indicates values differ from respective fed controls; ^*, p<0.05.