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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Aug 13;50(12):5837–5845. doi: 10.1167/iovs.09-3759

FIGURE 3.

FIGURE 3

Immunodetection of wild type and mutant CX50 in HeLa cells. (A) Aliquots from homogenates of untransfected HeLa cells and HeLa cells transfected with wild type CX50 or CX50G46V without induction and after induction with 1 µM ponasterone A for 48 hours were subjected to immunoblotting using anti-CX50 antibodies. No CX50 protein bands were detected in untransfected HeLa cells (HeLa). HeLa cells transfected with wild type or mutant CX50 (HeLa-CX50ind or HeLa-CX50G46Vind, respectively) showed a low level of CX50 protein expression before induction, but the CX50 protein levels increased markedly in both transfected cells after induction. The migration positions of the molecular mass standards are indicated. (B, C) HeLa-CX50ind and HeLa-CX50G46Vind cells were fixed 48 h after induction and subjected to immunofluorescence using anti-CX50 antibodies. Both HeLa-CX50ind (B) and HeLa-CX50G46Vind (C) showed a significant number of gap junctional plaques. Bar, 18 µm.