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Endosymbionts were detected in 5 (29%) of 17 Acanthamoeba isolates from domestic tap water using an orcein staining method. Arrow heads indicate endosymbionts. (A) Acanthamoeba castellanii Castellani. (B) Acanthamoeba sp. KA/WP3. (C) Acanthamoeba sp. KA/WP4. (D) Acanthamoeba sp. KA/WP8. (E) Acanthamoeba sp. KA/WP12. (F) Acanthamoeba sp. KA/WP9. Bar = 10 µm.
