Figure 1.

Evaluation of islet grafts early (days 4 and 12) and late (day 178) after intraportal islet transplantation. Four days (A, C, E) and 14 days (B, D, F) after islet transplantation, 70% of the liver was removed. Histological examination revealed multiple foci of necrosis on the liver surface (A, B). Hematoxylin/eosin staining (H&E) (C, D), and immunoperoxidase staining with antibody against insulin (magnification ×200) (E, F) of liver sections, are shown. Histological sections for each marker are representative of three animals from each treatment group. In recipients of 350 islets with long-term graft survival, 70% of the liver was also removed (G, H). Blood glucose levels of recipients were monitored, and normoglycemic mice underwent partial hepatectomy at the time points indicated by arrows in (I) to confirm long-term graft function and acceptance.