FIGURE 3.
Secondary plots showing the substrate concentration dependence of the apparent steady-state parameters kcatapp and kcat/Kmapp in the presence of 0. 5% cholate. Points represent the calculated kinetic parameters of initial rate versus substrate curves, and the fitted lines are described as follows: A, kcat = 3.5 ± 1.1 s−1 and Km-Zn = 1.6 ± 4.0 μm; B, kcat/Km-DIX = 0.08 ± 0.05 × 106 m−1 s−1 and KiZn = 11.4 ± 5.8 μm; C, kcat = 3.5 ± 1.1 s−1 and Km-DIX = 46.1 ± 26.7 μm; D, kcat/KmKi = 6.7 ± 5.6 × 109 m−2 s−1.