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. Author manuscript; available in PMC: 2010 May 12.
Published in final edited form as: Mol Cancer Res. 2009 May 12;7(5):634–644. doi: 10.1158/1541-7786.MCR-08-0371

Figure 3.

Figure 3

Partial localization of Syk to cell-cell contacts. A, Tet-inducible MCF7 cells plated on glass coverslips were treated with tetracycline to induce Syk-EGFP and examined by fluorescence microscopy. B, MCF10A cells transiently expressing Syk-EGFP were treated with EGTA for 20 min, washed and placed in calcium-containing medium for 0, 20 or 45 min. Cells were fixed, permeabilized and stained for E-cadherin using DECMA-1 and a fluorescently tagged secondary antibody. Cells were examined for localization of Syk-EGFP (green) and E-cadherin (red) by fluorescence microscopy. Images were overlayed to produce the pictures in the bottom row of panels. C, MCF7 cells expressing Syk-EGFP(K396R) were fixed and examined by fluorescence microscopy. The arrowhead illustrates the localization of a subset of Syk-EGFP(K396R) to the plasma membrane. D, Syk-EGFP was transiently expressed in Ras-transformed MCF10A cells, which were fixed and examined by fluorescence microscopy. The arrowhead illustrates the weak localization of a subset of Syk-EGFP to the plasma membrane. E, Western blotting of lysates from Ras-MCF10A cells (lanes 1 and 2) or MCF10A cells (lane 3) with an anti-Syk antibody. Bars = 10 μm.