Fig 4. Downregulation of EglN2 Suppresses Cancer Cell Proliferation.
(A and B). Immunoblot (A) and cell proliferation assay (B) of T47D cells infected with retrovirus encoding shRNA against EglN2 (sequence 1 or 4) or a scrambled control shRNA. In (B) cells were grown in Phenol Red-free RPMI medium supplemented with 5% charcoal/dextran treated FBS in the presence or absence of estrogen (10 nM) as indicated.
(C - F). Immunoblot (C and E) and cell proliferation (D and F) assay of BT474 (C and D) and ZR75-1 cells (E and F). Error bars = 1 SEM.