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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: Cancer Cell. 2009 Nov 6;16(5):413–424. doi: 10.1016/j.ccr.2009.09.029

Fig 6. Impaired Proliferation of Cells Lacking EglN2 is Due to Cyclin D1 Loss.

Fig 6

(A and B). Immunoblot (A) and cell proliferation (B) assay of T47D cells that were infected with retroviruses encoding shRNAs against EglN2 [sequence 1 or 4](or scrambled control) and then infected with a retrovirus encoding HA-Cyclin D1 (or empty vector).

(C and D). Immunoblot (C) and cell proliferation assay (D) of BT549 RB−/− breast carcinoma cells infected with retroviruses encoding shRNAs against EglN2 (sequence 1 or 4) or scrambled control shRNA. In (D) cells were grown in Phenol Red-free RPMI medium supplemented with 5% charcoal/dextran treated FBS in the presence or absence of estrogen (10 nM) as indicated.

(E and F). Immunoblot (E) and cell proliferation (F) assay of T47D cells that were infected with a retrovirus encoding E7 (or empty retrovirus) and then superinfected retroviruses encoding shRNAs against EglN2 [sequence 4](or scrambled control). Note that E7 promotes the degradation of pRB (Boyer et al., 1996) and that hyperphosphorylated and hypophosphorylated pRB are not resolved under these electrophoretic conditions.

Error bars = 1 SEM.