Skip to main content
. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Cell. 2005 Oct 7;123(1):89–103. doi: 10.1016/j.cell.2005.07.032

Fig. 2. Inhibition of G6P dehydrogenase induces apoptosis.

Fig. 2

A) Extracts supplemented with either dehydroisoandrosterone (DHEA) or buffer were analyzed for caspase 3 activity. Shown is a representative experiment repeated on 3 separate batches of oocytes with similar results. B) (upper): Extract was incubated at room temp and assayed for caspase activity. (middle): Samples withdrawn at 0 and 2 h of incubation were assayed for G6P levels by monitoring NADPH production over time (absorbance at 340 nm) in the presence of excess G6P dehydrogenase and NADP. (lower): Addition of excess G6P maintained NADPH production at all times tested. Dotted line indicates the fact that the scales are different in the extracts +/− G6P as NADPH was produced at much higher rates in the presence of excess G6P. C) Oocytes treated with DHEA or buffer are shown in representative micrographs. D) Percent survival of oocytes treated with buffer or DHEA. E) Cytochrome c release was measured in parallel by filtering aliquots of lysed oocytes through a 0.1μm microfilter and analyzing the filtrate by anti-cytochrome c immunoblotting. F) Percent survival of oocytes treated with buffer, DHEA or DHEA and cell permeable D-methyl malate.