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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Methods Enzymol. 2008;449:221–237. doi: 10.1016/S0076-6879(08)02411-7

Figure 2. pH dependence of aminoacyl-tRNA stability at 37 °C.

Figure 2

RNA isolated from a wild type strain under acidic conditions was incubated at 37 °C in pH 4.5 buffer (10 mM NaOAc pH 4.5, 10 mM MgCl2, 20 mM KCl) or pH 7.5 buffer (50 mM HEPES pH 7.5, 10 mM MgCl2, 20 mM KCl) for the indicated times, followed by ethanol precipitation and resuspension in 10 mM NaOAc pH 4.5, 1 mM EDTA. The RNA was then separated on an acidic gel and analyzed by Northern blotting.