Table 1.
Summary of tRNA species probed for aminoacylation.
| tRNA probed | Probe sequence | Targeted region | Separation on acidic gel | Reference |
|---|---|---|---|---|
| Arg(ICG) | TAGCCAGACGCCGTGAC | 18–34 | excellent | (Gu et al., 2005) |
| Ala(AGC) | CATGCTAAGGGAGCGCG | 23–39 | good | Baker, M., unpublished |
| Gly(GCC) | AAGCCCGGAATCGAACCGG | 50–68 | good | (Gu et al., 2005) |
| His(GUG) | ACTAACCACTATACTAAGA | 5–23 | good | (Gu et al., 2005) |
| mito-His(GUG) | CGAACTCAGATTTAACGCA | 38–57 | good | (Gu et al., 2005) |
| Ile(IAU) | GCACGGTGCCTTAACCA | 17–32 | good | Baker, M., unpublished |
| Leu(CAA) | AGATTCGAACTCTTGCATCTT | e3–62 | good | (Alexandrov et al., 2006) |
| Lys(UUU) | TAAAAGCCGAACGCTCTACC | 18–37 | good | (Gu et al., 2005) |
| iMet(CAU) | GCCCTGCGCGCTTCCA | 16–32 | good | (Alexandrov et al., 2006) |
| Met(CAU) | TTCAGATTATGAGACTGACGC | 24–44 | good | (Alexandrov et al., 2006) |
| Phe(GAA) | TGTGGATCGAACACAGGACC | 45–64 | good | Chernyakov, I., unpublished |
| Thr(IGU) | GGATTTGAACCGATGATCT | 44–62 | good | Baker, M., unpublished |
| Trp(CCA) | AGTCGAAAGCTCTACCAT | 15–33 | poor | Baker, M., unpublished |
| Val(AAC) | TGGTGATTTCGCCCAGGA | 59–76 | poor | (Alexandrov et al., 2006) |
| Tyr(GUA) | CGAGTCGAACGCCCGAT | 46–62 | poor | (Gu et al., 2005) |
| Pro(UGG) | GGAATTGAACCCAGGGCCTCTCGCA | 38–62 | very poor | Chernyakov, I., unpublished |
| Ser(IGA) | CGCCTTAACCACTCGGCC | 9–26 | very poor | Baker, M., unpublished |
| Cys(GCA) | AATCTGCTGCGCTACCACT | 14–33 | not detected | Chernyakov, I., unpublished |