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. Author manuscript; available in PMC: 2010 Mar 25.
Published in final edited form as: Chem Biol. 2009 Sep 25;16(9):990–1000. doi: 10.1016/j.chembiol.2009.08.012

Figure 2.

Figure 2

αIIbβ3 CHO K1 cell line. (A) CHO K1 cells do not express αIIbβ3 (top). Transfected αIIbβ3 CHO K1 cells express αIIbβ3 at the cell surface, as detected by anti-β3 and PAC-1 antibody (bottom). (B) cRGDFG, a cyclic peptide that specifically binds to α5 and αV integrins, inhibits CHO K1 cell adhesion to 0.5% GRGDSC SAMs. (C) CHO K1 cells adhere to 0.5% GRGDSC SAMs in the absence of inhibitors, but (D) fail to adhere to the same substrate in the presence of [cRGDFG]=300 µM. (E) αIIbβ3 CHO K1 cells adhere to 0.5% GRGDSC SAMs in the presence of [cRGDFG]=300 µM, (F) this interaction is nulled by the presence of PAC-1.