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. Author manuscript; available in PMC: 2010 Mar 25.
Published in final edited form as: Chem Biol. 2009 Sep 25;16(9):990–1000. doi: 10.1016/j.chembiol.2009.08.012

Figure 3.

Figure 3

αIIbβ3 CHO cell adhesion and cytoskeletal structure on model substrates. Optical micrographs of αIIbβ3 CHO cells incubated with [cRGDFG]=300 µM that adhered and spread on 0.5% SAMs presenting GRGDSC (A), HHLGGAKQAGDVC (B) and a mixed monolayer comprised of both peptides (C). As a control, αIIbβ3 CHO cells adhered and spread on a dodecanethiol monolayer with adsorbed Fbg (D). Scale bars are 300 µm. Immunofluorescence staining of αIIbβ3 CHO cells incubated with [cRGDFG]=300 µM that adhered and spread on 0.5% SAMs presenting GRGDSC (E), HHLGGAKQAGDVC (F), a mixed monolayer comprised of both peptides (G) and the Fbg control (H). Focal adhesions were visualized with an anti-vinculin antibody (red), actin stress fibers were visualized with AF 488 phalloidin (green), and nuclei were visualized with Hoescht (blue). Scale bars are 50 µm.