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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Cell Signal. 2008 Jul 2;20(10):1890–1899. doi: 10.1016/j.cellsig.2008.06.018

Figure 9. Gab2-dependent G-CSF-stimulated cell proliferation is inhibited by mutation of Gab2 tyrosine 643.

Figure 9

DT40GR cells stably transfected with vector alone (control), Flag-tagged Gab2 (wtGab2), the Y614F mutant (Gab2 Y614F), or the Y643F mutant (Gab2 Y643F), were cultured in triplicate in basal medium, or basal medium plus either G-CSF (+ G-CSF) or added serum (+ serum). The cells were pulsed with tritiated thymidine to assess new DNA synthesis; incorporation of radioactivity was determined by scintillation counting. Results are expressed as mean ± standard deviation. Compared to cells transfected with wild-type Gab2, G-CSF stimulated proliferation of cells expressing Gab2 Y643F was markedly inhibited (p < 0.01). Cells expressing Gab2 Y614F showed a smaller decrease in G-CSF-stimulated tritiated thymidine incorporation (p = 0.04). The presence of wild-type Gab2, or either of the Gab2 mutants, had no significant effect on serum-stimulated proliferation.