Fig. 3.
Both knockdown of AKAP350 (A) and stress (B) alter Golgi structure. HeLa cells were transfected with either non-specific scrambled RNA duplexes or siRNA duplexes specific for AKAP350A (A) or treated with 0.5 mM arsenite for 30 min (B), fixed and dual stained for AKAP350A (green in merged images) and cis-Golgi marker gm130 (red in merged images). (A) Reduction of AKAP350A expression altered Golgi apparatus morphology (dispersal of Golgi elements). (B) Arrows indicate intact Golgi structure with AKAP350 co-localization; arrowheads indicate partially displaced AKAP350A staining from Golgi to stress granules and Golgi disassembly. Bar = 10 μm.