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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Exp Cell Res. 2008 Dec 3;315(3):542–555. doi: 10.1016/j.yexcr.2008.11.011

Fig. 4.

Fig. 4

Nocodazole inhibits delivery of AKAP350A to stress granules. AKAP350A (green) and G3BP (red) localization in HeLa cells treated with arsenite in the absence (A) or presence (B) of nocodazole pre-treatment. Microtubule disassembly with nocodazole was confirmed with a–tubulin staining (see Supplemental Fig. S7). Bar = 10 μm. Enlarged images are shown in insets in the right corners. Note that nocodazole treatment resulted in a major separation of AKAP350A from G3BP-positive stress granules. (C) Quantification of AKAP350A and G3BP in stress granules based on size (μm) or the number of stress granules per cell demonstrates that nocodazole elicits greater amount of smaller G3BP-positive stress granules. Solid green bars: AKAP350 control; solid red bars: G3BP control; hatched green bars: AKAP50 nocodazole; hatched red bars: G3BP nocodazole. *p<0.001 vs AKAP350 staining in untreated; **p<0.001 vs G3BP staining in controls. The results are the mean±SE.

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