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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Exp Cell Res. 2008 Dec 3;315(3):542–555. doi: 10.1016/j.yexcr.2008.11.011

Fig. 9.

Fig. 9

RNA transcripts associated with stress granules. Enrichment of specific RNA transcripts in association with AKAP350A. RNAs were isolated from untreated and arsenite-treated HepG2 cells with either AKAP350A antibodies (α350) or preimmune rabbit serum (C). A random primed cDNA was prepared from each fraction and RNAs were detected by PCR amplification of 120 nt fragments with primers specific for calumenin, CSNK1A1 or ECT2 (30 cycles). The cDNA from HepG2 cell was used as a positive control. Calumenin, CSNK1A1 and ECT2 were only detected in the specific AKAP350A immunoisolates. Note that HSP27 mRNA was abundant in HepG2 cells, but was not associated with AKAP350A.

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