FIGURE 3.
Acsl1L−/− liver contained less acyl-CoA than control liver. Control and Acsl1L−/− mice were fed a 45% fat diet (HFD, Research Diets D12451) or a matched 10% fat control diet (SD, Research Diets D12450B) from 8 to 23 weeks of age. Liver samples were snap frozen after the mice were fasted for 4 h. Lipid intermediates were extracted and measured by tandem mass spectrometry. A, total long chain acyl-CoA and acyl-CoA species. B, total long chain LPA and LPA species. C, relative expression of Gpat isoform mRNA in Acsl1L−/− liver (n = 6 for each group). Total RNA was isolated from liver, and 20 μg was synthesized to cDNA for SYBR Green quantitative PCR. Target gene expression was normalized to the endogenous control β-actin and expressed as 2−ΔΔCT relative to the control. D, total DAG and DAG species. Data show means ± S.E. from 10 mice/group. B, *, p < 0.05 versus control mice (Student's t test). A and C, *, p < 0.05; **, p < 0.01 versus control mice fed the same diet (two-way ANOVA); #, p < 0.05; ##, p < 0.01 versus 10% fat control diet in the same genotype (two-way ANOVA).