FIGURE 4.

N-terminal sequencing confirms the identities of colonic α-defensins. A, contains the following samples electrophoresed in a non-reducing AU-PAGE gel: Crp3 (lane 1), Crp4 (lane 2), a preparation of α-defensins from complete ileum (lane 3), a preparation of α-defensins from complete colon (lane 4). Individual bands from the complete colon α-defensin preparation in lane 4 are labeled with lowercase letters a–e. Peptide bands were transferred to polyvinylidene difluoride membrane as preparation for 11 cycles of N-terminal sequencing (“Experimental Procedures”) with the resulting deduced peptide identities and primary structures shown in B. Underlined sequence represents amino acid residues determined by sequencing. Because residue position 10 is the first variable amino acid among full-length Crps 1–3 and 6, it is boxed to highlight the identities of individual peptides.