FIGURE 7.

Treatment with IL-1β abolished HDAC-1 and HDAC-2 binding to the promoter of PDGF-Rα. A, NIH-3T3 cells were pretreated with the HDAC inhibitor SAHA for 1 h prior to incubation with IL-1β (10 ng/ml) treatment for 4 h. Levels of PDGF-Rα mRNA were determined by RT-PCR (left) or qRT-PCR (right). * denotes p < 0.05 as compared with no treatment control using Student's t test. Error bars denote S.E. B, NIH-3T3 cells were pretreated with TSA for 1 h prior to exposure to IL-1β (10 ng/ml) for 4 h. mRNA was extracted, and RT-PCR was conducted. GAPDH is used to show unbiased loading. C, NIH-3T3 cells were incubated with IL-1β for 4 h prior to ChIP analysis with p65, HDAC-1, and HDAC-2 antibodies (Ab). TI, total input. No antibody (No Ab) was used as negative control. Western blotting (right) indicates that IL-1β had no inhibitory effect on HDAC-1 or HDAC-2 expression.