FIGURE 7.

Phosphorylation of BAD wild type and BAD mutants by C-RAF promotes association with 14-3-3 proteins. Interactions of BAD with 14-3-3ζ were monitored by the surface plasmon resonance technique. GST-BAD samples (200 pmol) were phosphorylated by highly activated C-RAF-R/L (10 pmol), as described under “Experimental Procedures.” Approximately 1000 response units of phosphorylated BAD were captured onto an anti-GST chip. 14-3-3ζ (200 nm) was injected, and the association-dissociation curves were monitored. For better understanding, we used here (and in Fig. 8) the mouse nomenclature.