TABLE 1.
Human BAD phosphopeptides obtained by trypsin and/or GluC digestion
The putative phosphorylation sites and the number of the phosphates detected in each of the peptides by mass spectrometry are indicated. ND, not determined.
| hBAD Peptide | Amino acid sequence | Phosphates/peptide | Phosphorylation site(s) |
|---|---|---|---|
| 21–36 | GLGPpSPAGDGPSGSGK | 1 | Ser-25 |
| 21–36 | GLGPSPAGDGPSGSGK | 1 | Ser-32 or Ser-34 |
| 73–94 | HSSYPAGTEDDEGMGEEPSPFR | 1 | Ser-74 or Ser-75 |
| 71–94 | SRHSSYPAGTEDDEGMGEEPpSPFR | 1 | Ser-91 |
| 73–94 | HSSYPAGTEDDEGMGEEPpSPFR | 1 | Ser-91 |
| 95–112 | GRpSRpSAPPNLWAAQRYGR | 2 | Ser-97 and Ser-99 |
| 97–109 | pSRSAPPNLWAAQR | 1 | Ser-97 |
| 97–109 | SRpSAPPNLWAAQR | 1 | Ser-99 |
| 99–109 | pSAPPNLWAAQR | 1 | Ser-99 |
| 116–126 | RMpSDEFVDSFK | 1 | Ser-118 |
| 117–126 | MpSDEFVDSFK | 1 | Ser-118 |
| 117–133 | MpSDEFVDpSFKKGLPRPK | 2 | Ser-118 and Ser-124 |
| 132–142 | PKpSAGTATQMR | 1 | Ser-134 |
| 134–142 | pSAGTATQMR | 1 | Ser-134 |
| 124–149 | SFKKGLPRPKSAGTATQMRQSSSWTR | 5 | ND |