FIGURE 9.

ChIP assay showing that Dlk1 is a direct target of TCF/LEF in fAEC II. A, two putative TCF/LEF binding sites (Dlk1-BS1, −1399 to −1392, and Dlk1-BS2, −974 to −967) of the Dlk1 promoter. The arrows indicate primers used for PCR amplification. B, the immunoprecipitated DNAs using anti-β-catenin antibodies were PCR-amplified using the primer pairs for Dlk1-BS1 and Dlk1-BS2. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cyclin D1 (CNND1) served as negative and positive controls, respectively. Normal IgG was used as an immunoprecipitation control.