FIGURE 2.

LANCL2 silencing abrogates ABA-induced functional effects in human granulocytes. After 24 h from transfection, untreated, negative control siRNA-transfected, or LANCL2 siRNA-transfected granulocytes were subjected to the following functional assays, following stimulation with 10 μm ABA: A, chemotaxis (gray bars) and chemokinesis (striped bars) assays; *, p < 0.05 by Tukey test, compared with negative control and untreated cells; B, measurement of ROS production; C, phagocytosis of fluorescent latex beads. *, p < 0.01 and #, p > 0.5 by t test, compared with the respective unstimulated cell type (n = 3).