FIGURE 8.

Staining of intact and permeabilized CD38⁺/LANCL2⁺ HeLa with anti-V5 mAb. 24 h after transfection with LANCL2 plasmid, cells were incubated for 6 h in the absence (a, b, and c), or in the presence (d) of 100 μm cycloheximide, to prevent protein synthesis. Subsequently, cells were fixed and either permeabilized (a, c, and d) or not (b) with 0.1% Triton X-100, and then stained with the anti-V5 peptide mAb, followed by Alexa 488 secondary antibody (green), while nuclei were highlighted in red with propidium iodide. Images are from one representative experiment out of three performed, yielding closely comparable results.