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Phosphoinositide dependence of PH1-Ank GAP activity. Reactions contained 0.03 nm PH1-Ank recombinant protein, myrArf5·GTP as a substrate, and LUVs comprising each of the different phosphorylated derivatives (2.5 μm) of phosphatidylinositol, a total phosphoinositide concentration of 50 μm, and a total phospholipid concentration of 500 μm. The averages from three experiments ± S.E. are presented.
