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. 2009 Aug 7;284(41):28069–28083. doi: 10.1074/jbc.M109.028266

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Role of PH domains for PtdIns(3,4,5)P₃-independent GAP activity. A, activity with LUVs lacking PtdIns(3,4,5)P₃. PH1-Ank and ArfGAP-Ank were titrated into reaction mixtures containing myrArf5·GTP and 500 μm phospholipids but no PtdIns(3,4,5)P₃. B, activity in the absence of lipids or detergents. PH1-Ank and ArfGAP-Ank were titrated into reactions containing [L8K]Arf5·GTP. The average ± S.E. from three experiments is presented.