FIGURE 6.
Thr305/306 autophosphorylation in the presence of Ca2+. Thr305/306 autophosphorylation time course estimated by Western blotting. 1 μm αCaMKII was allowed to autophosphorylate for the indicated lengths of time as described under “Materials and Methods.” Aliquots were taken at the indicated time points; the reaction was quenched by pipetting the aliquots taken into SDS sample buffer. Shown are Western blots and respective densitometric analyses of representative time courses at 21 (A and D), 30 (B and E), and 37 °C (C and F). Care was taken that the intensity of the bands was in the quasilinear range of densities (for details, see Fig. 3). Densitometric analysis of Western blots was carried out as described under “Materials and Methods.”