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Effect of MG132 treatment of HeLa and PC12 cells on c-Jun and c-Fos expression. HeLa or PC12 cells were untreated (−) or treated (+) with 10 μm MG132 for 12 h. Total cells extracts were analyzed by immunoblots with the indicated antibodies to detect total c-Fos and c-Jun and the phosphorylation of c-Jun (P-c-Jun) as described under “Experimental Procedures.”
