FIGURE 10.

Ischemia/reperfusion injury induces autophagy with concomitant increase in FoxO3 activation in mouse hearts. FVBN mice were subjected to 40 min of ischemia plus 30 min of reperfusion (I/R) and compared with sham. A, Western blot analysis of heart homogenates with quantification by densitometric analysis in B. Increased levels of LC3II (2.1-fold) and the ratio of LC3II/LC3I (2-fold) in I/R indicates induction of autophagy in ischemia/reperfusion injured mouse heart. Decreased phosphorylated p-FoxO3 (∼45%) was observed in I/R injured mouse compared with sham control indicating increased activity of FoxO3 in I/R. No significant change in the level of p-FoxO1 was observed between the two groups. The level of Sirt1 was also increased (2-fold) in ischemia/reperfusion injured mouse hearts compared with sham control. B, densitometric and statistical analysis of the Western blot shown in A. Significance (*) was determined by Student's t test (p < 0.05; sham (n = 4) and I/R (n = 6).